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AIM: To explore the effect of bone marrow-derived mesenchymal stem cells (BMSCs) combined with vitamin E on the inflammatory reaction in acute kidney injury ( AKI) rats.METHODS:Gentamicin was used to in-duce AKI and the rats were treated with BMSCs combined with vitamin E.After treatment, the rat plasma and kidney tis-sues were collected, and the expression of inflammatory factors at mRNA and protein levels was detected by real-time quan-titative PCR and ELISA.RESULTS:After the treatment with BMSCs combined with vitamin E, the inflammatory proteins were down-regulated in the plasma and the renal tissues.Compared with single treatment group, the decreases in the in-flammatory proteins were more obvious in combined treatment group.CONCLUSION: The method of BMSCs combined with vitamin E takes the anti-inflammatory effect on AKI, indicating a new and potential mode in clinical application for AKI therapy.
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Objective: To investigate the applied feasibility of scaffold with modified PLA (Polymer of lactic acid) in tissue engineering. Methods:First, we adopted salting-in method to prepare porous foam scaffold. Then, we reconstructed tissue engineering skin by epidermal cells and fibroblasts combined with modified PLA. On the 14th day of cell culturing in vitro, we was a control. Results:The arfificial skin is composed of epidermis and dermis and similar to natural skin in appearance. The skin consists of fibroblasts and keratinocytes, which are in various proliferation and differentiation stages. Fibroblasts and keratinocytes distribute on the surface of polymer of lactic acid (PLA) and the number of fibroblast and keratinocyte increase. Conclusion:Dialdehyde starches (DAS) not only improve the function of PLA but also have good effects on cells. Moreover, it does not affect the growth and the metabolism of the cells. So it is feasible to use modified scaffold to construct tissue engineering skin.
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BACKGROUND: The extracellular matrix (ECM) in demis consists of collagen, elastin and other matrix components, and as an in vitro skin model, the tissue-engineered artificial skin should have the synthesis, excretion and decomposition functions. OBJECTIVE: To observe the collagen and proteoglycan metabolism of tissue-engineered artificial skin. DESIGN: Single sampling observation SETTING: Department of Dermatology, Peking University Shenzhen Hospital. MATERIALS: Siderophilin, trypsin, 3H-proline and sirius red, etc. METHODS: The experiment was conducted at Department of Dermatology, Peking University Shenzhen Hospital and Institute of Frontier Medical Science, Jilin University from June 2000 to December 2004.①The tissue-engineered artificial skin was prepared in accordance with literatures.②The collagen synthesis of tissue-engineered artificial skin was examined by 3H proline incorporation. The culture plate was added with 3H-proline in each hole and cultured for 4 hours respectively in the 1st, 2nd and 3rd weeks after tissue engineering culture, and compared with natural skin.③Picric acid sirius red staining was applied to observe collagen synthesis and excretion functions of tissue-engineered artificial skin, while proteoglycan synthesis and excretion were examined by AB-PAS staining respectively after 1, 2, 3, 4 and 6 weeks of culture. MAIN OUTCOME MEASURES: ①The detections of collagen synthesis and excretion; ②The detections of proteoglycan synthesis and excretion; ③ The results of 3H-proline incorporation experiment. RESULTS: ①The quantity of 3H-proline incorporation of tissue-engineered artificial skin was similar with that of normal skin after 7 and 14 days of culture, but obviously higher than that of normal skin at the 21st day after culture. ②Under polarization microscope, the tissue-engineered artificial skin presented fasciculate red double refraction and thin green double refraction, whereas simple matrix lattice only displayed red double refraction.③AB-PAS staining was negative in tissue-engineered artificial skin after 1 and 2 weeks culture together with simple matrix lattice, while positive in artificial skin after 3-6 weeks of culture. CONCLUSION: The tissue-engineered artificial skin has the capacity of synthesizing and excreting ECM collagen and proteoglycan.
ABSTRACT
BACKGROUND: Skin transplantation is the most effective conventional method to cure large area full-thickness skin damage caused by empyrosis or some diseases, but present deficiency of skin donator is the largest barrier in front. The most ideal way to solve this problem is to use tissue-engineering skin reconstructed by self-skin cells as seed cells.OBJECTIVE: To investigate the effects of tissue engineered artificial animal skin in animal grafting experiment.DESIGN: A randomized controlled trial SETTING: Institute of frontier medical sciences and department of dermatology in a university.MATERIALS: Study was performed in the Cell-Engineering Institute of Jilin University from September 1998 to July 2001. Totally 20 newborn Wistar rats and 24 8-week old male nude mice were selected into our study.METHODS: Full-thickness wounds(diameter: 20 nn) were made on the backs of twenty-four nude mice to establish full-thickness skin defect animal model for grafting by the tissue-engineered reconstructive artificial skin made by chitosan(CH) as stromal scaffold. Twenty-four 8-week old nude mice were divided into artificial skin (AS) group, chitosan membrane(CH) group and control group (CG). All wounds were covered with AS, CH or petrolatun gauze correspondingly. The wounds of each groups were observed daily,techniques like histology and infrared-ray scan were used for a dynamical surveillance on the 3rd, 7th, 14th and 21st days.MAIN OUTCOME MEASURES: ① general observation; ② blood supply in recipient area under infrared-ray observation; ③ histological observation.RESULTS: Transplanted AS had a favorable fusion between tissue-engineered skin and self-skin on the 3rd day after grafting with a few of ingrowths of capillaries. The color of the AS was closed to self-skin. The capillaries gradually increased in the grafts over time. The new epidermis was clearly consisted of stratum basale, stratum spinosum, stratum granulosum, and stratum corneum. Keratinization enhanced with exfoliation. Cells in dermis increased and the scaffold gradually degraded. The secreted extracellular matrix increased as well. On the 14th day after grafting, the wounds almost recovered.The color of the grafted artificial skin was more similar to the nature skin with very little scaring, which indicated that a second grafting was unnecessary. The scabs did not completely fall off in CH group until the 14th day, and the wound was not healed. The color was darker than that of AS group. The scabs fell off in CG, and the wounds were big and deep with dark red color.CONCLUSION: The new reconstructive tissue-engineered artificial skin with CH as stromal scaffold has good histocompatibility, which can be applied in grafting for full-thickness wounds.